AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

Figure 2

(a) and (b): SDS-PAGE electrophoretic profiles of 6 weeks glycation of BSA (100 mg/mL) at 37^˚C in PBS 10 mM pH 7.4; lanes 1 and 3: BSA + 1.6 D-glucose (AGE-modified BSA) (loaded with 10 μg and 5 μg protein, resp.); lanes 2 and 4: unglycated BSA (control) (loaded with 10 μg and 5 μg protein, resp.); lane 5: molecular weight marker (MWM 105 Bio-Rad). This was carried out using a 4% stacking and 7.5% resolving gel and Coomassie blue staining. (c) Corelation MW-RF.

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