AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

Figure 3

FPLC separation of glycated BSA on Superdex 200 HR 10/30 column, 155 μg protein/100 μL injection volumes: 6 weeks incubation of BSA (100 mg/mL) at 37^˚C in 10 mM PBS pH 7.4; curve (1) BSA + 1.6 M D-glucose (AGE-modified BSA); curve (2) unglycated BSA (control).