AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

Figure 5

The relative expression ratios (𝑅) for procollagen 12 and for procollagen 31 genes after 24 hours incubation with AGE-BSA of culture human dermal fibroblasts. 𝑅=𝐸^target^{ΔCTtarget(control-sample)}𝐸_ref^{ΔCTref(control-sample)},(2) was expressed in arbitrary units. The data are shown as the mean ± SD for two independent experiments run in triplicate each time with significant differences compared to control (5 mg/mL BSA) at ^*P < .05 and ^***P < .01.