AGEs and Glucose Levels Modulate Type I and III Procollagen mRNA Synthesis in Dermal Fibroblasts Cells Culture

Table 1

Sequences of human primers used for real-time PCR.

Gene (mRNA)	Oligonucleotide primer sequence (5^′-3^′)	Amplification fragment	Annealing temperature (^˚C)
			Calculate	Use
Procolagen 1 2 (PCol 12) sense	GTGGTTACTACTGGATTGACC	331	53.4	54
Procolagen 1 2 (PCol12) antisense	TTGCCAGTCTCCTCATCCAT
Procolagen 3 1 (Pcol31) sense	GGAGTAGCAGTAGGAGGAC	91	54	54
Procolagen 1 1 (PCol31) antisense	AACCAGGATGACCAGATGTA
18S RNA sense	CTCAACACGGGAAACCTCAC	133	53.5	54
18S RNA antisense	TTATCGGAATTAACCAGACAAATCG