Glinide, but Not Sulfonylurea, Can Evoke Insulin Exocytosis by Repetitive Stimulation: Imaging Analysis of Insulin Exocytosis by Secretagogue-Induced Repetitive Stimulations
Figure 1
Histogram of the numbers of fusion events (/200 m2) at 1-minute intervals after stimulation. Cells were perifused with 16.7 mM glucose (N = 16) (a), 0.5 M mitiglinide (N = 17) (b), and 0.5 M glimepiride (N = 11) (c) for 12.5 minutes. After the first stimulation, cells were perifused with 4 mM glucose for 15 minutes and stimulated again by the same secretagogues as in the first stimulation. Left and right columns show exocytotic responses during thefirst and second stimulations, respectively. (d) The numbers of total fusion events during the first and second stimuli. Data are represented as mean S.E.M per 200 m2.
