Glinide, but Not Sulfonylurea, Can Evoke Insulin Exocytosis by Repetitive Stimulation: Imaging Analysis of Insulin Exocytosis by Secretagogue-Induced Repetitive Stimulations
Figure 2
responses to repetitive stimulations. Cells were perifused with 4 mM glucose and then stimulated with 16.7 mM glucose (a), 0.5 M mitiglinide (b), or 0.5 M glimepiride (c) for 12.5 minutes. After the first stimulation, cells were perifused with 4 mM glucose for 15 minutes and stimulated again by the same secretagogues as in the first stimulation. Data are represented as a ratio of the fluorescence intensity expressed as F/, where is the ratio of 340 to 380 nm at the baseline. (mean + S.E.M., N = 9, 7, and 7 for 16.7 mM glucose, mitiglinide, and glimepiride, resp.).
