Altered Regulation of Contraction-Induced Akt/mTOR/p70S6k Pathway Signaling in Skeletal Muscle of the Obese Zucker Rat

<table>Contraction-induced mTOR (Ser 2448) phosphorylation is altered with insulin resistace. The basal (control) and contraction-induced phosphorylation of the mTOR in Plantaris muscles from lean (LNZ) and obese (OSXZ) Zucker rats at 0, 1, and 3-hours after HFES. Phosphorylation of mTOR was determined by immunoblotting for phosphorylation on Ser2448. Relative abundance represents ratio of phosphor to total protein. An asterisk (<svg height="13.75" id="M27" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 7.7375002 13.75" width="7.7375002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,.062,5.525)"><path d="M471 153q-22 -15 -61 -13q-25 31 -45.5 49.5t-56.5 41.5q4 -71 28 -134q-17 -33 -42 -46q-24 12 -42 46q24 63 28 134q-36 -23 -56.5 -41.5t-45.5 -49.5q-36 -2 -61 13q0 28 19 59q65 10 130 43q-65 33 -130 43q-19 31 -19 59q22 15 61 13q25 -31 45.5 -49.5t56.5 -41.5
q-4 71 -28 134q17 33 42 46q24 -12 42 -46q-24 -63 -28 -134q36 23 56.5 41.5t45.5 49.5q36 2 61 -13q0 -28 -19 -59q-65 -10 -130 -43q65 -33 130 -43q19 -31 19 -59z" id="x2217"></path></g>
</svg>) indicates significant difference (<i>P</i> &lt; .05) from the control animals and a dagger (<svg height="15.8875" id="M28" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 6.5749998 15.8875" width="6.5749998" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.012,-0,0,-.012,.062,7.662)"><path d="M268 -96h-46q2 45 1 111q0 203 -29 267q30 55 33 123q-82 -3 -127 -32q-33 13 -52 47q20 35 53 47q43 -28 126 -32q-2 51 -9.5 78.5t-28.5 64.5q22 42 56 61q33 -17 56 -61q-21 -37 -28.5 -64.5t-9.5 -78.5q85 4 127 32q13 -5 28.5 -18.5t23.5 -27.5q-19 -34 -52 -48
q-47 29 -127 32q3 -63 34 -123q-31 -69 -31 -267q0 -75 2 -111z" id="x2020"></path></g>
</svg>) indicates significant difference (<i>P</i> &lt; .05) from corresponding time points across the animal models.</table>

Journal of Diabetes Research

fig3

Figure 3

Figure 3: Altered Regulation of Contraction-Induced Akt/mTOR/p70S6k Pathway Signaling in Skeletal Muscle of the Obese Zucker Rat 