Murine Pancreatic Beta TC3 Cells Show Greater 2^′,5^′-Oligoadenylate Synthetase (2^′5^′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-𝛼 or Poly(I:C) Treatment than Pancreatic Alpha TC3 Cells

Figure 2

Enzyme activity of 2^′5^′AS in non-stimulated and IFN-α stimulated beta TC3 and alpha TC3 cells. Beta TC3 (a) and alpha TC3 (b) cells were incubated in the presence or absence of 500 U/ml of IFN-α. After the indicated time periods, cell lysates were prepared. The lysates were incubated with 0.15 μCi α ³²P-ATP for 120 minutes at 30^∘C in the presence of 100 𝜇g/mL poly(I:C), and then separated by ascending TLC. The generated 2^′5^′A were quantitated by PhosphorImager analysis. Enzyme activity was expressed in units per milligram (𝑛=4, standard deviations varied between 6–13%).

(a)

(b)