Murine Pancreatic Beta TC3 Cells Show Greater 2^′,5^′-Oligoadenylate Synthetase (2^′5^′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-𝛼 or Poly(I:C) Treatment than Pancreatic Alpha TC3 Cells

Figure 5

Protein expression and activity of 2^′5^′AS in beta TC3 and alpha TC3 cells transfected with mouse IFN-α4-containing vector and poly(I:C). Beta TC3 and alpha TC3 cells were transiently transfected with pcDNA3.1-mIFN-α4, poly(I:C), and pcDNA3.1-mIFN-α4 plus poly(I:C), respectively. pcDNA3.1 empty vector was used as a negative control. After 24 hours, cell lysates were prepared. Immunoblot (a) and 2^′5^′AS activity assay (b) were performed as described under the materials and methods.

(a)

(b)