Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells

Figure 3

NADPH oxidase subunit p22^phox expression. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for 24 hours or 48 hours, or pretreated with 10 𝜇M rosiglitazone (RSG), or 10 𝜇M ciglitazone or 10 𝜇M troglitazone) and p22^phox was detected by immunoblotting. Pretreatment with three different PPAR𝛾 agonists blocked HG-induced p22^phox expression (𝑛=5, ^∗𝑃<.05 versus NG, ^∗∗𝑃<.05 versus HG 24 hours or HG 48 hours).