Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells
Figure 7
Compound C reversed rosiglitazone prevented high glucose-induced reactive oxygen species generation . Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) for 3 hours or 48 hours, and then pretreated with 10 M rosiglitazone (RSG) or 50 M compound C (CPC), or combination of both, for 1 hour. Reactive oxygen species (ROS) generation was measured in DCF-loaded cells using confocal fluorescence imaging. RSG prevented high glucose stimulated ROS production that was abolished by coincubation with CPC ( cells, versus NG in the absence of RSG and/or CPC, versus HG in the absence of RSG and/or CPC). Magnification bar = 25 M.
