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Experimental Diabetes Research
Volume 2010, Article ID 173652, 6 pages
http://dx.doi.org/10.1155/2010/173652
Methodology Report

Comparison of Measurements of Autoantibodies to Glutamic Acid Decarboxylase and Islet Antigen-2 in Whole Blood Eluates from Dried Blood Spots Using the RSR-Enzyme Linked Immunosorbent Assay Kits and In-House Radioimmunoassays

1Unit for Diabetes and Celiac Disease, Department of Clinical Sciences, Lund University, Wallenberg Laboratory, Entrance 46, University Hospital MAS, 205 02 Malmö, Sweden
2Department of Clinical Chemistry, University Hospital MAS, 205 02 Malmö, Sweden
3Unit for Diabetes and Celiac Disease, Department of Clinical Sciences, Lund University, Clinical Research Center (CRC), University Hospital MAS, 205 02 Malmö, Sweden

Received 26 June 2009; Revised 7 September 2009; Accepted 24 March 2010

Academic Editor: Rodica Pop-Busui

Copyright © 2010 Anders Persson et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To evaluate the performance of dried blood spots (DBSs) with subsequent analyses of glutamic acid decarboxylase (GADA) and islet antigen-2 (IA-2A) with the RSR-ELISAs, we selected 80 children newly diagnosed with type 1 diabetes and 120 healthy women. DBSs from patients and controls were used for RSR-ELISAs while patients samples were analysed also with in-house RIAs. The RSR-ELISA-GADA performed well with a specificity of 100%, albeit sensitivity (46%) was lower compared to in RIA (56%; ). No prozone effect was observed after dilution of discrepant samples. RSR-ELISA-IA-2A achieved specificity of 69% and sensitivity was lower (59%) compared with RIA (66%; ). Negative or low positive patients and control samples in the RSR-ELISA-IA-2A increased after dilution. Eluates from DBS can readily be used to analyse GADA with the RSR-ELISA, even if low levels of autoantibodies were not detected. Some factor could disturb RSR-ELISA-IA-2A analyses.