Peroxynitrite Mediates Diabetes-Induced Endothelial Dysfunction: Possible Role of Rho Kinase Activation
Figure 2
FeTPPs blocked diabetes-induced oxidative stress and nitrotyrosine formation in rat vessels. Effect of diabetes on oxidative and nitrative stress in rat aortic homogenate was determined by lipid peroxides using MDA assay (a) and nitrotyrosine immunoreactivity using slot blot (b). Both lipid peroxides and nitrotyrosine were significantly increased in diabetic vessels. Treatment with FeTTPS blocked these effects in diabetic () but not in treated controls (). Values are expressed as means ± SEM, (n = 6 in each group, versus control). (c). Effect of FeTPPs on DCF fluorescence. Bovine aortic endothelial cells (BAEC) were incubated with normal (NG, 5 mM) and high glucose (HG, 25 mM) for 3 days (n = 5 in each group). High glucose-increased ROS formation, which was prevented by cotreatment with FeTPPs. Values are expressed as means ± SEM, versus control.
