Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study
Figure 4
Neutrophils in wound tissue. (a) Expression of lipocalin mRNA in nonwounded back skin and wound tissue isolated from CD-fed, HFD-fed or ob/ob mice. The time after injury is indicated. * as indicated by the bracket. Each single experimental time point depicts the mean ± SD obtained from 12 wounds () isolated from 4 individual animals (). (b) Immunoblots showing the presence of Ly6G protein in nonwounded (ctrl) and wounded (1 to 11 d wd) skin in CD, HFD and ob/ob mice as indicated. Each time point depicts 8 wounds from () from 4 individual mice (). A Ponceau S (PonS) staining of the immunoblots is shown to control equal loading. (c) Sections from 7-day wound tissue isolated from CD and HFD mice were stained for neutrophil-specific Ly6G protein (brown colour) as indicated. Ly6G-positive signals were indicated by arrows. The epithelial margins are indicated by a yellow line. Scale bar = 500 μm. gt, granulation tissue; ne, neoepithelium; sc, scab; we, wound margin epithelia.
