Glucose Regulation of Thrombospondin and Its Role in the Modulation of Smooth Muscle Cell Proliferation
Figure 7
TSP-1 degradation. (a)–(e) SMCs grown to confluency in medium containing 25 or 5 mM glucose were incubated overnight in SFM and then treated with (+) a cathepsin inhibitor (100 nM), (+) bafilomycin (100 nM) or vehicle () for 6 hours prior to extracellular matrix preparation and immunoblotting with the anti-TSP-1 antibody or cell lysis/immunoprecipitation with the antibodies indicated. The graphs show the results of arbitrary scanning units derived from the western immunoblots of three independent experiments. Vertical line indicates where discontinuous bands from the same gel were used.
