Insulin Receptor Substrate 2 Expression and Involvement in Neuronal Insulin Resistance in Diabetic Neuropathy
Figure 2
IRS2 protein expression pattern in murine lumbar DRG. Fluorescence immunohistochemistry was used to examine IRS2 expression in adult C57Bl/6 mouse lumbar DRG. ((a) and (d)) Photomicrographs of IRS2 immunoreactivity in DRG neurons. IRS2 was expressed in most neurons of the DRG in mice. ((b) and (e)) Photomicrographs of the same sections stained with antibodies to NF-200 (b), which labels large, myelinated neurons in the DRG or peripherin (e), which labels unmyelinated small DRG neurons. ((c) and (f)) Merged images of IRS2 and NF-200 (c) labeling illustrate that many IRS2-positive neurons also express NF-200, suggesting that many IRS2-positive neurons are large myelinated neurons. Similarly, merged images of IRS2 and peripherin (f) labeling illustrate that many IRS2-positive neurons coexpress peripherin, suggesting that many IRS2-positive neurons are small unmyelinated neurons. Scale bar = 100 μm.
