Insulin Receptor Substrate 2 Expression and Involvement in Neuronal Insulin Resistance in Diabetic Neuropathy
Figure 5
Diabetes decreases insulin-stimulated Akt activation in DRG neurons. Primary cultures of lumbar DRG neurons from ob/ob diabetic mice and nondiabetic controls were stimulated with 100 nM insulin for 15 minutes and harvested for Western blot. Membranes were probed for activated Akt (pSer(473)Akt) and normalized to total Akt levels. Insulin significantly increased Akt activation in both nondiabetic and diabetics cultures (*). In contrast, insulin-stimulated Akt activation in cultures from diabetic ob/ob mice was significantly suppressed in comparison to nondiabetic controls (†). There were no significant differences in baseline pAkt levels between nondiabetic and diabetic mice. * and † denote ; for nondiabetic without insulin, for nondiabetics with insulin, for diabetics without insulin, and for diabetics with insulin.
