Insulin Receptor Substrate 2 Expression and Involvement in Neuronal Insulin Resistance in Diabetic Neuropathy
Figure 6
Diabetes decreases insulin-stimulated neurite outgrowth in DRG neurons. Primary cultures of lumbar DRG neurons from diabetic ob/ob and nondiabetic mice were grown in either insulin-free media or media containing 100 nM insulin for 5 days. Neurites were then stained with SMI 312, a pan-axonal marker. Neurite area was quantified using a stereological grid applied overtop photomicrographs of cultured wells. Photographs of representative wells are shown on the top panel: (a) nondiabetics without insulin (), (b) diabetics without insulin (), (c) nondiabetics with insulin (), and (d) diabetics with insulin (). (e) Quantification of neurite outgrowth in the different treatment groups. Insulin significantly increased neurite outgrowth in nondiabetic animals, whereas there was no effect of insulin on neurite outgrowth in neurons from diabetic mice. * and † denote . Scale bar = 100 μm.
