Osmolarity and Glucose Differentially Regulate Aldose Reductase Activity in Cultured Mouse Podocytes
Figure 3
In podocytes cultured in normal glucose (NG), 5-day exposition to hyperosmolar conditions (Hosm) noticeably increased and redistributed AR immunostaining (b), as compared to the cells from normoosmolar (NG-Nosm) (a) or high glucose (HG-Hosm) (c) conditions. In the NG-Nosm cells, immunoreactivity was localized predominantly in perinuclear regions (arrows), while in NG-Hosm cells, intensive staining was diffuse throughout the cytoplasm (arrows). HG-Hosm cells stained to AR in a diffuse manner, with less pronounced perinuclear regions as compared to NG-Nosm. In response to high osmolarity, rearrangements of F-actin cytoskeleton were observed in NG cells. Thick transcellular stress fibers observed in NG-Nosm cells (d) were replaced by thin, more delicate F-actin filaments (e). Nonspecific staining control (f) was prepared as described in Materials and Methods. Magnification ×400 (a–c, f) and ×200 (d, e).
