Increased Expression of the Tail-Anchored Membrane Protein SLMAP in Adipose Tissue from Type 2 Tally Ho Diabetic Mice
Figure 1
Expression of GLUT-4 in adipose tissue in Tally Ho mice. Adipose tissue from both NG and HG Tally Ho mice was isolated and analyzed by immunoblotting with anti-GLUT-4 (a). The expression of the GLUT-4 was quantified by densitometry and is shown as bar graph (b). Real-time PCR for GLUT-4 message normalized to β-actin from both NG and HG Tally Ho mice (c). Subcellular membrane fractions Cyto (cytosolic fraction) and Mem (membrane fraction) from adipose tissue in both NG and HG Tally Ho mice were analyzed by immunoblotting with anti-GLUT-4 (d). The expression of GLUT-4 from cytosolic and membrane fractions was quantified by densitometry and is shown as bar graph (e). The data are expressed as mean ± SEM. 4 to 6 mice were included in each group. *Significantly different () .
