Assessment of Glucagon-Like Peptide-1 Analogue and Renin Inhibitor on the Binding and Regulation of GLP-1 Receptor in Type 1 Diabetic Rat Hearts

Table 2

The calculated dissociation constants (𝐾_𝑑) and binding affinity constants (𝜏) of glucagon-like peptide-1 (GLP-1) with its receptor at the cardiomyocytes (N = 8).

Rat group	𝐾𝑑 (moles × 10^-15)	𝜏 (min)
Normal (N)	0.64 ± 0.021^a	0.34±0.005^a′
Diabetic (D)	0.33 ± 0.018^b	0.6±0.018^b′
Diabetic + Insulin (DI)	0.47 ± 0.019^c	0.43±0.009^c′
Diabetic + Exendin-4 (DE)	0.59 ± 0.025^d	0.41±0.0084^d′
Diabetic + Aliskiren (DA)	0.12 ± 0.014^e	1.37±0.0938^e′
Diabetic + Exendin-4; + Aliskiren (DEA)	0.077 ± 0.01^f	1.63±0.13^f′
Diabetic + Insulin; + Aliskiren (DIA)	0.19 ± 0.027^g	1.33±0.08^g′

Cardiomyocytes: dissociation constants significant at 𝑃<.05 for (b, c), (b, g), (c, d), (e, f), (f, g) and 𝑃<.001 for (a, b), (a, c), (a, e), (a, f), (a, g), (b, d), (b, e), (b, f), (c, e), (c, f), (c, g), (d, e), (d, f), (d, g), (f, g). The other comparative values are not significant (𝑃>.05). Binding affinities significant at 𝑃<.001 for (a′, b′), (a′, c′), (a′, d′), (a′, e′), (a′, f′), (a′, g′), (b′, c′), (b′, d′), (b′, e′), (b′, f′), (b′, g′), (c′, e′), (c′, f′), (c′, g′), (d′, e′), (d′, f′), (d′, g′). The other comparative values are not significant (𝑃>.05).