Angiotensin II Type II Receptor Deficiency Accelerates the Development of Nephropathy in Type I Diabetes via Oxidative Stress and ACE2

Figure 6

Basal expression of intrarenal RAS genes (i.e., Agt, AT₁R, ACE, and ACE2) in freshly isolated RPTs from WT (open bar) and AT₂RKO (black bar) male mice at the age of 16 weeks. (a) RT-qPCR. Quantitation of relative intra-renal RAS genes was normalized to their own β-actin mRNA. The y-axis shows the percentage of relative values compared to WT animals (100%). *P ≤ 0.05. (b) Western blotting. Quantitation of relative densities of intrarenal RAS genes was normalized to β-actin. The y-axis shows the percentage of relative values compared to WT animals (100%). **P ≤ 0.01.

(a) mRNA basal expression in RPT by qRT-PCR.

(b) Basal protein expression in RPT by western blot.