High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells
Figure 3
High glucose increases MT-1 mRNA and MT-1/-2 protein expression. mProx24 cells were serum-starved for 24 h before stimulation with high glucose or mannitol. (a) Cells were harvested after 24 h, and MT-1 mRNA expression was analyzed by qRT-PCR in three independent experiments and normalized for GAPDH. (b–e) MT-1/-2 protein expression was determined by immunofluorescent staining with anti-MT-1/-2 antibody 24 h after stimulation followed by densitometric analysis. Results are means ± SEM of three independent experiments. * versus high glucose; NG: normal glucose; Man: mannitol; HG: high glucose. Scale bar: 100 μm.
