High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells
Figure 4
Vitamin E suppresses high glucose-induced MT-1/-2 expression. mProx24 cells were serum-starved and pretreated with vehicle or vitamin E for 24 h before stimulation with high glucose or mannitol. MT-1/-2 expression was determined by immunofluorescent staining. MT-1/-2 expression was not increased by mannitol (b) compared with normal glucose (a), but was increased by high glucose (c). High glucose-induced MT-1/-2 expression was attenuated by vitamin E pretreatment in a dose-dependent manner (d: 20 nM; E: 100 nM; F: 200 nM). The cells depicted are representative of three independent experiments. (g) Densitometric quantification of MT-1/-2 immunofluorescence. Results are means ± SEM of three independent experiments. * versus high glucose; NG: normal glucose; Man: mannitol; HG: high glucose; Vit E: vitamin E. Scale bar: 100 μm.
