TRAIL and DcR1 Expressions Are Differentially Regulated in the Pancreatic Islets of STZ- versus CY-Applied NOD Mice
Figure 4
DcR1 expression analysis in STZ- or CY-treated NOD mice. Pancreata were isolated at days 0, 2, 4, 7, 11, and 14 in the 150 mg/kg STZ-injected group and at days 0, 1, 4, 7, 14, and 21 in the 200 mg/kg CY-injected group. Representative images are shown for negative, weak, moderate, and strong stainings for DcR1 expression. (a) Sample stainings for STZ-treated mice are displayed through (A)–(D), and for CY-treated mice through (E)–(H): negative staining for DcR1 is shown in (A) and (E); moderate IC staining is evident in some acinar cells in (E), depicted by arrow. (B) Weak IC positivity in the islet, moderate IC staining in the acinar cells, (C) weak IC, moderate nuclear (thin arrow) and strong nuclear (thick arrow) staining in the islet, moderate nuclear staining in acinar cells, (D) weak IC and strong nuclear positivity in the islet, along with strong nuclear positivity in acinar cells, (F) weak IC staining in the islet, moderate IC positivity in some acinar cells, (G) moderate (thin arrow) and strong (thick arrow) positivity in the islet, no staining in acinar cells, (H) moderate IC, as well as strong nuclear stainings in the islet, moderate nuclear staining in the acinar cells, (I) control staining with secondary antibody alone. Smaller islets are depicted at 400x magnification for clarity. Other images were taken at 200x. (b) Immunohistochemical staining evaluations for DcR1 were performed as described in Section 2. Each bar represents mean data from three mice. Increase at day 14 in the STZ group is significant (). Error bars represent ±SEM. IC: intracytoplasmic.
