Table of Contents Author Guidelines Submit a Manuscript
Experimental Diabetes Research
Volume 2012 (2012), Article ID 168368, 4 pages
http://dx.doi.org/10.1155/2012/168368
Methodology Report

A Protocol for Measurement of Noncoding RNA in Human Serum

1O’Brien Institute, 42 Fitzroy Street, Fitzroy, VIC 3065, Australia
2Department of Surgery, St. Vincent’s Hospital, University of Melbourne, Melbourne, VIC, Australia
3Faculty of Health Sciences, The Australian Catholic University, VIC, Australia
4DNA Sequencing Facility, Microbial Collection Center and National Center for Cell Science, Ganeshkhind Road, Pune MH 411007, India
5Cardiovascular Research Institute, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029-6574, USA
6NHMRC Clinical Trials Centre, The University of Sydney, Rm 316, Level 1, Medical Foundation Building, 92 Parramatta Road, Camperdown, NSW 2050, Australia

Received 27 February 2012; Accepted 26 April 2012

Academic Editor: Anandwardhan Hardikar

Copyright © 2012 Caroline J. Taylor et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs. Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease. Currently, there are many methods of isolating total RNA from liquid samples. Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.