A Protocol for Measurement of Noncoding RNA in Human Serum

Table 1

	μL/reaction	No. of reactions	Volume to add (μL)
10X RT buffer				0.5	12	6.25
100 nM dNTPs	0.05	12	0.625
RNase inhibitor	0.03	12	0.375
Nuclease-free water	1.42	12	17.75
Reverse transcriptase	0.33	12	4.125
Total volume (μL)	2.33