Differential Proteome Profiling Using iTRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients

Table 3

Differentially expressed proteins by 2-DE in microalbuminuria versus normoalbuminuria.

Gene name^a	Accession number^b	Up-/down-regulated	Mol. Mass, Da (pI)^c	Peptides matched	Total ion C.I.%^d	Ma/Na; (2-DE)^f	Ma/Na (iTRAQ)^g
ALB	P02768	Up	71317.2 (5.92)	2	100.00	50.8±15.3	3.09
ALB	P02768	Up	71317.2 (5.92)	2	99.84	17.5±4.0	3.09
ALB	P02768	Up	71317.2 (5.92)	2	100.00	14.2±2.5	3.09
HSPG	P98160	Down	468527.5 (6.06)	2	100.00	0.28±0.041	0.68
FABP	Q01469	Down	15496.7 (6.6)	1	97.76	0.27±0.037	0.29
MASP2	Q9UMV3	Down	75684.6 (5.47)	2	100.00	0.10±0.005	0.29
AMBP	P02760	Down	38974 (5.95)	2	99.91	0.20±0.002	1.44
RTEL1	Q9NZ71	Up	152278.2 (8.68)	1	96.77	3.0±0.5	—
FBLN5	Q9UBX5	Down	50146.7 (4.58)	1	99.36	0.12±0.007	—

^a-bGene name from the Expasy database correspond to protein accession number. ^bAccession numbers represent entries in the Human CDS database. ^cMolecular mass (mol. mass) is presented by Da, while isoelectric point stands for pI. ^dTotal ion score and total ion CI % for MALDI-TOF/TOF were calculated using GPS v3.5 in the MASCOT search program (v2.0). ^f-gRatio of differentially excreted protein for 2-DE and iTRAQ in microalbumiuric versus normoalbuminuric urines, respectively. Data are expressed as the mean ± SD.