Glibenclamide Induces Collagen IV Catabolism in High Glucose-Stimulated Mesangial Cells

Figure 4

MMP2 mRNA, protein expression, and gelatinolytic activity in rat mesangial cells after chronic exposure (8 weeks) to high glucose with and without treatment with glibenclamide. Gelatinolytic activity was measured in 24- and 48-hour conditioned media. Protein and gelatinolytic activities are expressed relative to control. Zymography results were expressed as total amount of MMP2 determined by the sum of the area under both MMP2 and pro-MMP2 bands. mRNA 𝑛=12, protein 𝑛=4, zymography 𝑛=12, per experimental group.