Rising Intracellular Zinc by Membrane Depolarization and Glucose in Insulin-Secreting Clonal HIT-T15 Beta Cells

Figure 4

Intracellular zinc responses to glucose stimulation in HIT-T15 beta-cells. (a) Images of beta-cells loaded with 5 μM FluoZin-3 AM before and after 10 mM glucose application. Scale bars are 10 μm. (b)–(e) Heterogeneous increases of intracellular zinc in the presence of stimulating glucose (10 mM): early onset response ((b); 𝐍=𝟖), late onset response ((d); 𝐍=𝟐), oscillation- or wave-like responses ((c), (e); 𝐍=𝟑). The line above traces indicates the continuous presence of 10 mM glucose.

(a)

(b)

(c)

(d)

(e)