L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry
Figure 1
Representative chromatograms of D- and L-lactic acid derivatives(a). (a) Internal standard [13C3]-L-lactate chromatogram (435 μmol/L, 72 pmol). (b) Representative chromatogram of a standard solution of D- and L-Lactate (351 μmol/L, 58.2 pmol, 501 μmol/L, and 83.0 pmol, resp.) and molecular structures of optical isomers L- and D-lactates. (c) Representative chromatogram of a urine sample (D- and L-Lactate; 14.1 μmol/L, 2.3 pmol, 74.6 μmol/L, and 12.4 pmol, resp.). (d) Representative chromatogram of a plasma sample (D- and L-Lactate; 11.2 μmol/L, 1.9 pmol, 1375.0 μmol/L, and 227.7 pmol, resp.). (a)Injection volume: 2 μL.
