L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry

Table 3

Recovery data for plasma and urine.

Plasma	Mean (SD) μmol/L	CV, %	Recovery, %
D-lactate added^(a); μmol/L (𝑛=5)
0	8.1 (0.2)	2.9	—
52.7	67.3 (1.2)	1.8	112.4
105.4	127.7 (3.7)	2.9	113.5
L-lactate added^(a); μmol/L (𝑛=5)
0	1365 (44.6)	3.3	—
1504	2931 (26.2)	0.9	104.2
3008	4443.2 (125.1)	2.8	102.3
Urine^(b)	Mean (SD) μmol/mmol creatinine	CV, %	Recovery, %
D-lactate added^(a); μmol/L (𝑛=5)
0	0.91 (0.03)	3.4	—
98.8	8.03 (0.10)	1.3	110.9
197.6	14.34 (0.29)	2.0	104.7
L-lactate added^(a); μmol/L (𝑛=5)
0	4.84 (0.13)	2.6	—
94	10.99 (0.38)	3.5	100.6
188	16.56 (0.14)	0.8	96.0

^(a)Addition of 25 μL standard solution to 25 μL plasma or urine.
^(b)Creatinine concentration: 15.4 mmol/L.