Time-Dependent Alterations in Rat Macrovessels with Type 1 Diabetes
Figure 6
Mitochondrial membrane disruption in aorta. Mitochondria were graded on the basis of membrane intactness with grade 1 as a completely intact organelle. Grade 2 indicated disruption of the outer mitochondrial membrane. Grade 3 indicated disruption of the inner mitochondrial membrane, and grade 4 meant that both membranes were disrupted. In the SMCs from control animals, the mitochondria were predominantly intact throughout the course of the study. In contrast, in the SMCs from the diabetic animals there were more grade 2–4 mitochondria present at day 50 and the percentage of disrupted mitochondria grew over the 150 day diabetes duration until over half of the mitochondria determined to be grade 3-4. Grade 3 measurements were more rare and shown as a thin sliver in the diabetic 150-day figure ( and 1046 mitochondria for 50-day control and diabetic respectively; and 425 for 100-day control and diabetic groups; and 446 for 150-day control and diabetic groups).
