Metformin Stimulates FGF21 Expression in Primary Hepatocytes

Figure 3

Compound C abolishes the effect of metformin on FGF21 expression. (a) FGF21 mRNA, (b) FGF21 protein, and (c) G6Pase mRNA expression in primary rat hepatocytes incubated for 24 hrs with Compound C in presence of 1000 μM metformin. Data are means ± SEM; ^∗𝑃<0.05, ^∗∗𝑃<0.01 versus 0 μM Compound C treated-hepatocytes, ^#𝑃<0.05, ^##𝑃<0.01 versus nontreated hepatocytes, analyzed by paired Student’s t-test, 𝑛=3–5.

(a)

(b)

(c)