A PEDF-Derived Peptide Inhibits Retinal Neovascularization and Blocks Mobilization of Bone Marrow-Derived Endothelial Progenitor Cells
Figure 1
PEDF-34 acts in a concentration-dependent manner to inhibit cell proliferation and induce apoptosis specifically in endothelial cells. (a) The purity of primary BRCEC was examined using Dil-Ac-LDL uptake assays. The cells were counter-stained with DAPI, and exhibited a >99% purity based on positive Dil-Ac-LDL uptake. Both rat Müller cells (b) and BRCEC (c) were treated with increasing concentrations of PEDF 34-mer ranging from 25 to 400 nM. Viable cells were quantified after 72 h by MTT assay and expressed as % of the cells in control treated with the BSA only (denoted as NT on graph). (d) BRCECs were treated with increasing concentrations of PEDF 34-mer for 24 h. Apoptotic cells were quantified by counting Annexin V positive cells using FACS and expressed as % in total cells (mean + SD, ).
