A PEDF-Derived Peptide Inhibits Retinal Neovascularization and Blocks Mobilization of Bone Marrow-Derived Endothelial Progenitor Cells
Figure 5
Characterization of circulating Tie2-GFP+ cells as EPCs. (a) Tie2-GFP+ cells from Tie2-GFP mice were sorted onto slides and immunostained for the EPC-specific markers CD117 and CD133 and counterstained with DAPI. Confocal microscopy was used to capture images at 40x magnification; (b) EPCs were fixed, sorted for GFP, then immunostained for CD117, and subjected to FACS. Most Tie2-GFP+ cells stained positive for CD117, indicating the majority of circulating endothelial cells are EPCs.
