Mannan-Binding Lectin in Diabetic Kidney Disease: The Impact of Mouse Genetics in a Type 1 Diabetes Model

Table 2

Renal gene expression.

	ANOVA
mRNA, strain	𝑃MBL×D	𝑃D	𝑃MBLknockout
TGF-𝛽, C57BL/6JBomTac	NS	2.3-fold (1.7;3.1), 𝑃<0.001	NS
TGF-𝛽, 129S6/SvEvTac	NS	2.5-fold (1.8;3.6), 𝑃<0.001	44% (2;104), 𝑃=0.04
CTGF, C57BL/6JBomTac	NS	33% (9;64), 𝑃<0.008	−34% (−19;−46), 𝑃<0.001
CTGF, 129S6/SvEvTac	NS	2.2-fold (1.5;3.1), 𝑃<0.001	NS
Fibronectin, C57BL/6JBomTac	NS	2.6-fold (1.9;3.7), 𝑃<0.001	−30% (−2;−50), 𝑃=0.04
Fibronectin, 129S6/SvEvTac	NS	3.6-fold (2.4;5.5), 𝑃<0.001	NS
Collagen IV𝛼1, C57BL/6JBomTac	a	𝑃<0.03^b	NS^𝑐
Collagen IV𝛼1, 129S6/SvEvTac	NS	2.4-fold (2.1;2.8), 𝑃<0.001	16% (0;34), 𝑃=0.04
VEGF-A, C57BL/6JBomTac	NS	−51% (−22;−69), 𝑃=0.004	NS
VEGF-A, 129S6/SvEvTac	NS	−24% (−6;−38), 𝑃=0.02	NS
VEGFR-2, C57BL/6JBomTac	NS	NS	−30% (−14;−43), 𝑃=0.002
VEGFR-2, 129S6/SvEvTac	NS	NS	41% (12;77), 𝑃=0.005
Nephrin, C57BL/6JBomTac	NS	NS	NS
Nephrin, 129S6/SvEvTac	NS	NS	NS

Expression of mRNA measured by RT-PCR relative to 18S mRNA.
𝑃_MBL×D: interaction between diabetes and MBL.
𝑃_D: diabetic versus nondiabetic animals.
Values indicate diabetes compared with control (95% confidence interval). 𝑃_MBL: MBL knockout versus WT animals. Values indicate MBL knockout compared with wild-type (95% confidence interval).
NS: 𝑃>0.05.
a: values could not be fitted to normality and therefore Wilcoxon rank-sum tests were used to test effects of diabetes and MBL.
b: 𝑃=0.025 (WT versus diabetic WT, by Wilcoxon rank-sum tests) and 𝑃=0.028 (MBL-KO versus diabetic MBL-KO, by Wilcoxon rank-sum test).
c: WT versus MBL-KO by Wilcoxon rank-sum test.
One diabetic WT was omitted from analyses for technical reasons.