miR-7 putative promoters induce reporter activation. (a) Schematic representation of conserved sequences, upstream of pre-miR-7a-2 hairpins (“block 1” and “block 2”) and pre-miR-7b (“block 3”) that were subcloned upstream of pGL3 basic luciferase reporter. (b) Relative activation of luciferase firefly to renilla in HEK-239 cells, transfected with various reporters. pGL3-basic serves as control (“Ctrl”). (c) Relative luciferase activation in MIN6 beta cells transfected with the indicated reporters. experiments, in triplicates. Error bars represent ± SEM.
