Transactivation of miR-7 reporters in HEK 293T. (a) The luciferase activity of a reporter driven by “block 1” did not demonstrate response to Ngn3 or NeuroD/Beta2. However, reporters driven by “block 2” are induced by both Ngn3 and NeuroD/Beta2 (b). Highest luciferase response is induced by a combination of Ngn3 and NeuroD/Beta2. (c) “Block 3” transcription is transactivated only by NeuroD/Beta2 but not by Ngn3. Luciferase activity normalized to A20-Renilla expression and to transactivation by a control pcDNA3-empty vector. experiments, in triplicates. Error bars represent ±SEM (**).
