Inhibition of Protein Tyrosine Phosphatase Improves Angiogenesis via Enhancing Ang-1/Tie-2 Signaling in Diabetes

Figure 5

Inhibition of PTP promotes Ang-1-induced suppression of caspase-3 activation and increases cell survival under HG conditions. (a) Caspase-3 activity assay showing that exposure of MHMEC to Ang-1 significantly attenuates HG-induced caspase-3 activation; pretreatment of MHMEC with OV before the Ang-1 treatment further enhances the effect of Ang-1 on caspase-3 activity under HG conditions (𝑛=3–5 cell lines, ^#𝑃<0.05 versus NG, *𝑃<0.05 versus HG). (b) MTT assay for the cell survival showing that pretreatment with Ang-1 increases endothelial cell survival; pretreatment with OV before the Ang-1 treatment further enhances the effect of Ang-1 on endothelial cell survival under HG conditions (𝑛=3–5 cell lines, ^#𝑃<0.05 versus NG, *𝑃<0.05 versus HG).

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