A Comparison of Inflammatory and Oxidative Stress Markers in Adipose Tissue from Weight-Matched Obese Male and Female Mice
Figure 2
Adiponectin mRNA expression in gonadal adipose tissue is reduced by obesity in male mice but not weight-matched obese female mice. Male and female C57BL/6 mice were fed either a standard rodent chow or a high-fat diet (HFD) from 6 weeks of age until the HFD-fed group achieved a body weight of 45 g. At that time, both chow- and HFD-fed males (21 weeks old) or females (38 weeks old) were sacrificed and qRT PCR performed on gonadal adipose tissue. Relative mRNA expression of adiponectin was reduced by obesity in male mice, while obesity had no effect on adiponectin expression in female mice (a). Consistent with an obese phenotype, mRNA expression of leptin was elevated in both male HFD and female HFD mice (b). Data are reported as mean ± SE; –8 per group; means with different superscripts differ by .
