Ampicillin-Improved Glucose Tolerance in Diet-Induced Obese C57BL/6NTac Mice Is Age Dependent
Figure 2
(a) Oral glucose tolerance (mean and SEM, relative values). Statistics were calculated on areas under the curves (AUC) and showed statistically significant differences between groups 1 (Ampicillin+/DIO+) and 2 (Ampicillin−/DIO+) at five weeks of age (). At 11 weeks of age, no difference between the high-fat fed groups could be demonstrated, but differences between groups 1 (Ampicillin+/DIO+) and 3 (Ampicillin−/DIO−) were now evident (). At 16 weeks of age, differences between the early Ampicillin-treated group 1A (Ampicillin 5w+ 16w−/DIO+) and the low-fat fed group 3 (Ampicillin 5w− 16w−/DIO−) were still evident (). (b) At five weeks of age, no difference between body weights could be demonstrated, whereas differences between the high-fat fed groups 1 (Ampicillin+/DIO+) and 2 (Ampicillin−/DIO+) compared to the low-fat fed group 3 (Ampicillin−/DIO−) were evident at 11 weeks of age (). At 16 weeks of age, only a difference between the nontreated high-fat fed group 2B (Ampicillin 5w− 16w−/DIO+) and low-fat fed group 3 (Ampicillin 5w- 16w−/DIO−) could be demonstrated () (mean and SEM depicted). (c) Glycated hemoglobin (% HbA1c, mean and SEM) shows differences between the high-fat fed groups 1 (Ampicillin+/DIO+) and 2 (Ampicillin−/DIO+) at six weeks of age (), whereas now difference could be demonstrated at 12 weeks of age. At 17 weeks of age, differences were found between the early treated groups 1A (Ampicillin 5w+ 16w−/DIO+) and 1B (Ampicillin 5w+ 16w+/DIO+) and the late treated group 2A (Ampicillin 5w− 16w+/DIO+), respectively (; ).
