Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA: Possible Role in Proliferative Diabetic Retinopathy
Figure 1
PDR-aqueous humor stimulates cell migration in a proNGF-dependent manner. HRE cells were grown to confluence and then scratched using a standard cell scrapper. Cells were switched to serum free medium and treated with aqueous humor samples (10 μL/mL) in the presence or absence of either anti-proNGF antibody or rabbit IgG (1 μg/mL). Representative micrographs for wounded HME cells are shown after 18 hours of various treatments. Statistical analysis showed that aqueous humor increased mean cell migration by 1.8-fold compared to the control group. Addition of anti-proNGF antibody to aqueous humor samples significantly reduced the mean percent of cell migration to the level of the control group whereas IgG did not significantly impact stimulatory effect of aqueous humor samples. Addition of anti-ProNGF antibody to control HRE cells did not significantly affect percent cell migration compared to control group (*,‡statistically significant compared to control and aqueous humor groups, resp., (), of aqueous humor samples = 7, of cell cultures = 14–16).
