Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells
Figure 6
The expression of histone H2A and H2B ubiquitination in GMCs by immunofluorescent staining and laser scanning confocal microscopy applications. A1, NC group, B1, HG3 group, C1, MI group, D1, OP group, E1, negative control, and A2, B2, C2, D2, and E2 represent the corresponding groups of nuclear stains. (a) uH2A protein and (b) uH2B protein. uH2A and uH2B proteins were detected in the nucleus as green fluorescence overlapping with the blue fluorescence emitted by the nuclear stains DAPI. The uH2A protein was not detected in the NC group, but it was prominent in the HG3 group. The protein was strongly detected in the MG132 intervention group, which did not significantly change as a result of high osmotic pressure. The results for the uH2B protein were in contrast to the uH2A results.
