Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells

<table>The mRNA levels of uH2A, uH2B, and TGF-<i>β</i> in GMCs induced by high glucose and inhibited by MG132. (a) RT-PCR strip chart. (b) The relative gray value statistics graph of the mRNA level. Compared with the NC group, the mRNA levels of uH2A and uH2B were not different between the groups. The expression of TGF-<svg height="16.6625" id="M57" style="vertical-align:-3.56265pt" version="1.1" viewbox="0 0 10 16.6625" width="10" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> mRNA was greatly increased in the HG3 group and decreased in the MI group compared to the HG3 group. <svg height="13.95" id="M58" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 66.324997 13.95" width="66.324997" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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q0 -76 10 -138.5t37 -107.5t69 -45q32 0 55.5 25t35.5 68.5t17.5 91.5t5.5 105t-5.5 105.5t-18 92t-36 68t-56.5 24.5z" id="x30"></path></g><g transform="matrix(.017,-0,0,-.017,46.058,13.688)"><path d="M113 -12q-24 0 -39.5 16t-15.5 42q0 24 16 40.5t40 16.5t40 -16.5t16 -40.5q0 -26 -16 -42t-41 -16z" id="x2E"></path></g><g transform="matrix(.017,-0,0,-.017,49.934,13.688)"><path d="M241 635q53 0 94 -28.5t63.5 -76t33.5 -102.5t11 -116q0 -58 -11 -112.5t-34 -103.5t-63.5 -78.5t-94.5 -29.5t-95 28t-64.5 75t-34.5 102.5t-11 118.5q0 58 11.5 112.5t34.5 103t64.5 78t95.5 29.5zM238 602q-32 0 -55.5 -25t-35.5 -68t-17.5 -91t-5.5 -105
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</svg> versus NC group and <svg height="15.775" id="M59" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 67.387497 15.775" width="67.387497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> versus 1 HG3 group.</table>

Journal of Diabetes Research

fig8

Figure 8

Figure 8: Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells 