Boldine Prevents Renal Alterations in Diabetic Rats
Figure 6
Boldine reduces the increase in Etd uptake induced by high glucose + cytokines in mesangial cells. (a) The graph shows time-lapse measurements of fluorescence intensity of Etd bound to nucleic acids of mesangial cells cultured for 48 h under normal glucose (white triangle), high glucose + cytokines (TNF-α (10 ng/mL) and IL-1β (10 ng/mL)) (black circles), and high glucose + cytokines treated with 100 μM Boldine for the last 24 h (grey square). (b) Each bar represents the mean value of the rate of Etd uptake obtained from time-lapse curves. Mean ± SEM for 4 independent experiments. Normal glucose (White bar), high glucose + cytokine (black bar), or high glucose + cytokine with 100 μM boldine (grey bar) for 24 h; * using one-way ANOVA test followed by Bonferroni’s post hoc test.
