Variations in Rodent Models of Type 1 Diabetes: Islet Morphology
Figure 5
Islet size and cell composition in NOD mice. (a) Islets from NOD mouse that did not develop hyperglycemia were immunostained for β-cells (green), α-cells (red), and δ-cells (blue). Scale bar—100 μm for all images. (b) Islet from an NOD mouse diabetic for 1 week. (c) Islet from an NOD mouse diabetic for 3 weeks. (d) Because the onset of hyperglycemia was extremely variable in the NOD mice, islet diameter was plotted according to the duration of diabetes. Islet diameter decreased with increasing duration of diabetes. (e) Cell composition was also plotted according to the duration of hyperglycemia. With increasing duration of diabetes, β-cells were lost and α-cell numbers increased. for both graphs = 11.803 cells from 116 islets from 6 mice.
