Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60
Figure 6
Characterization of signaling pathways involved in Hsp60-mediated release of inflammatory mediators from C57BL/6J, NOD, and NZO mouse-derived (pre-)adipocytes. Cells from C57BL/6J (light grey bars), NOD (dark grey bars), and NZO mice (black bars) were exposed to Hsp60 after preincubation (1 h) with specific inhibitors against the MAP-kinase ERK1/2 (PD98059) (a, c, e) and NFκB (SN50) (b, d, f). After 24 h, IL-6 (a, b), KC (c, d), and MCP-1 (e, f) concentrations were determined in the culture supernatants by multiplex-beads-assay. The results show residual cytokine levels in percent of the cytokine concentrations released from cells incubated in the absence of Hsp60. Data show means + SEM from three to six experiments for each inhibitor. *, **, *** compared to incubation with Hsp60 alone set as 100%.
