Adipocytes from New Zealand Obese Mice Exhibit Aberrant Proinflammatory Reactivity to the Stress Signal Heat Shock Protein 60

Table 1

Inhibition of Hsp60 binding to C57BL/6J and NOD mouse-derived preadipocytes by antibodies directed against defined Hsp60 regions.

Anti-human Hsp60 antibody		Hsp60* binding (%)
Clone	Recognized Hsp60 region (aa)	C57BL/6J preadipocytes	NOD preadipocytes
H1	1–50	54.5±7.8^∗	46.2±11.9^∗
24/HSP60	1–200	25.5±0.6^∗∗∗	28.0±6.1^∗∗∗
H-300	274–573	62.7±5.2^∗	65.5±1.8^∗
4B9/89	335–366	70.5±7.5	78.8±7.9
HSPD1	383–419	70.5±1.7^∗∗∗	74.4±1.7^∗
LK-1	383–447	63.8±1.8^∗	53.6±12.4
K-19	523–573	61.9±4.0^∗∗∗	42.1±7.1^∗

The binding of (fluorescent-labeled) Hsp60* (100 nM) in the absence of antibodies was set as 100%. The data represent residual Hsp60 binding in the presence of antibodies as mean ± SEM of three independent experiments. The application of adequate isotype controls to rule out unspecific inhibitory effects, showed no significant inhibition, whereas inhibition > 50% is regarded as biologically relevant, statistically significant inhibition of Hsp60* binding is indicated as ^∗𝑃<0.05; ^∗∗∗𝑃<0.001.