The Class I Histone Deacetylase Inhibitor MS-275 Prevents Pancreatic Beta Cell Death Induced by Palmitate

Figure 2

Effects of MS-275 on the expression the ER stress markers. (a) The mRNA of Atf3/ATF3 and Chop/CHOP was quantified by quantitative real-time PCR from MIN6 cells and isolated human islets cultured with 0.5 mM palmitate plus MS-275 (grey bars) or DMSO (open bars) for 48 hrs. The mRNA levels were normalized against the Rplp0/RPLP0 and the expression levels from cells cultured with BSA (−) were set to 100%. Data are the mean of ± SEM of 3 independent experiments (; ). (b) For western blotting analysis, nuclear proteins were prepared from cells cultured for 48 hrs with 0.5 mM palmitate plus DMSO (−) or 1 μM MS-275. Immunoblotting was done using the anti-Atf3, anti-Chop, and anti-Tbp as the control. The figure shows the result of a representative experiment out of three. The data are the mean ± SEM of 4 independent experiments ().

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